Wenlin Huang
Ph.D., Professor


Institute of Microbiology
Chinese Academy of Sciences
Tel : 86-10-64807808
E-mail: wl_huang@im.ac.cn
Research Area
The group’s work focuses on the molecular mechanisms of the interactions between virus and host cells, the cellular transcriptional profiling in virus infection and the development of gene therapy drug.

Education & Professional Experience
Principle Investigator, PhD (Princeton University, USA, 1996).
Assistant Professor,Neurobiology Department, Allegheny University(1996-1997)
Group Leader, Molecular Virology Department, Advanced Viral Research. Yonkers (1997-2006)
Visiting Professor, Molecular Biology, Princeton University, Princeton(1997-2006)
Associate Editor in Chief, Chinese Journal of Cancer(1997-2006)
Chairman, CAST-Bio and Pharmaceutical Society, USA(1997-2006)
Professor, PI., Cancer Center, Sun Yat-sen University(2001-2005)
Honorary Professor of The Chinese University of Hong Kong (2006-2008)
Professor, PI., Institute of Microbiology Chinese Academy of Science.(2006-now)

Current Research
1. Recombinant adenovirus for gene therapy
Human adenoviruses are molecular parasites that rely on cellular mechanisms for expression of their reporter gene in formation. Recombination of foreign genes like endostation, angostetion, neurotrophin in to adenovirus can express efficiently with eukaryotic in target tissues for treating diseases. We have developed the injection of an adenovirus encoding human endosatin, and now the Phase 2 clinical trials are performed.
2. The unusual transcriptions in adenoviruses infection
We have established that typical RNA polymerase Ⅱ promoter is also transcribed by RNA polymerase Ⅲ in adenoviruses infected cells. The properties of RNA polymerase Ⅲ transcription, suggest that recognition of the E2E promoter of polymerase Ⅲ may serve to switch initiator, or ignite a threshold for RNA polymerase Ⅱ transcription. In addition, to investigate the mechanisms by which viral mRNA species are distinguished from their cellular counterparts for export to the cytoplasm during the late phase of subgroup C adenovirus infection, we have examined the metabolism of several cellular and viral mRNAs in human cells productively infected by adenovirus type5 (Ad5).
3. Minicircle DNA for antitumor gene therapy
Minicircles are superior to standard plasmid in terms of biosafety, improved gene transfer, and potential bioavailability. However, minicircle vectors have never been applied in antitumor gene therapy. One of huang’s works has suggested that minicircle-mediated IFNg gene transfer is a promising novel approach in the treatment of human nasopharyngeal carcinoma (NPC).
4. The pathogenesis of avian influenza virus infections
  The biological basis for the severity of human H5N1virus has remained unclear. Studies suggested that the virulence of H5N1viruses may be caused in part by the functions of NS1 and NS2 proteins. We study the role of the two proteins in the regulation of cellular cytokines and signal transduction pathway.

 

Publications:

 

Molecular Virology     Graduate teaching materials
Signal Transduction    People`s Medical Publishing House

 

1. Xia Xiao…Huang, W. Induction of cell Cycle Arrest and Apoptosis in Human Nasopharyngeal Carcinoma Cells by ZD6474, an Inhibitor of VEGFR Tyrosine Kinase with Additional Activity against EGFR Tyrosine Kinase. International Journal of cancer, 2007. Accepted (Corresponding author) (IF:4.693)
2. Hongli Li…Huang, W. Pharmacokinetic and Pharmacodynamic Study of Intratumoral Injection an Adenoviral Encoding Endostatin in Patients with Advanced Cancer. Gene Therapy. 2007. In printing. (Corresponding author) (IF:4.782)
3. Huang BJ…Huang, W. Long-term Toxicity Studies in canine of E10A an Adenoviral Vector Mediated Human Endostatin Gene. Hum Gene Ther, 2007 18(3):207-21(Corresponding author) (IF:4.514)
4. Xue G…Huang, W. Dendritic cells modified with 6Ckine/IFNgamma fusion gene induce specific cytotoxic T lymphocytes in vitro. Cancer Immunol Immunother. 2007 May 15(Corresponding author) (IF:4.313)
5. Lin X, ...Huang, W ., Jiang W. A Phase I Clinical Trial of an Adenovirus-Mediated Endostatin Gene (E10A) in Patients with Solid Tumors. Cancer Biol Ther. 2007 May 13; 6(5) (Corresponding author) (IF:2.818)
6. Wu J...Huang, W .Minicircle-IFNgamma induces antiproliferative and antitumoral effects in human nasopharyngeal carcinoma. Clin Cancer Res. 2006 Aug 1;12(15):4702-13(Corresponding author) (IF:6.177)
7. Li L…Huang, W.. Adenovirus-mediated intra-tumoral delivery of the endostatin gene inhibits tumor growth in nasopharyngeal carcinoma. Int J Cancer. 2006, 118(8): 2064-71 (Corresponding author) (IF:4.693)
8. Liang Z …Huang, W. Bioactivity and stability analysis of endostatin purified from fermentation supernatant of 293 cells transfected with Ad/rhEndo.
Protein Expr Purif. 2007 Dec;56(2):205-11 (Corresponding author). (IF:1.867)
9. Zhao P …Huang, W. Adenovirus-mediated delivery of human IFNgamma gene inhibits prostate cancer growth. Life Sci. 2007 (Corresponding author). (IF:2.389)
10. Zhao P…Huang, W. E10A, an adenovirus carrying human endostatin gene, in combination with docetaxel treatment inhibits prostate cancer growth and metastases. Journal of Cellular and Molecular Medicine. 2007 (Corresponding author) Accepted.(IF:6.555)
11. Jialing Huang… Wenlin Huang …Hui Zhang. Cellular microRNAs contribute to HIV-1 latency in resting primary CD4+ T lymphocytes. Nature Medicine. 200713(10):1241 (IF:28.588)

Patents
(1) Adenovirus carrying human endostatin gene for cancer gene therapy. Ch patent No:ZL 01127894.3
(2) Adenovirus carrying human interferon gamma gene for immunotherapy in cancer patients. Ch patent No:03126707.6
(3) Inhibitor for tyrosine kinase receptor in treatment of cancer and synthetic methods.       CN1542004A
(4) Adenovirus carrying human spike gene of SARS for vaccination. Ch patent o:03126634.7
(5) Vaccination against SARS by nucleic acid method. Ch patent No:03126635.5
(6) Assay method for determining product R’s effect on adenovirus infection of HeLa cells. United States Patent: 6440658
(7) E7 gene expression of nucleoside polypeptide inhabitting HPV-16. US Patent: .4204701
(8) Recombinant human IFN-g harbored in adenovirus and preparation method. PCT/CN2004/000427
(9) A preparation method for quinoline derivative and application on anti-tumor drug. PCT/CN2005/000660
(10) Tyrosine kinase inhibitor, its preparation method and application as an anti-tumor drug. PCT/CN2005/000661
(11) Quinoline derivative as an anti-tumor drug and its preparation method. PCT/CN2005/000663.
(12) Tyrosine kinase receptor inhibitor as an anti-tumor drug and its preparation. PCT/CN2005/000662

Laboratory Staff
Shijuan Gao     Assistant Professor     gaoshj@im.ac.cn
Wei Jiang        Research Associate     jiangw@im.ac.cn
Jie Zhao       Technician
Bin Ge         Technician
Xiaofeng Feng Postdoctoral Fellow
Liping Song   Postdoctoral Fellow
Students
Zhenzhu Zhang  2006-2009
He Liu           2006-2009
Chao Zhang     2007-2010
Qingtao Wang   2008-2011