Wenjun (Frank) Liu
Ph.D., Professor

 

Center for Molecular Virology
Institute of Microbiology
Chinese Academy of Sciences
Tel : 86-10-64807367
E-mail: liuwj@im.ac.cn

Brief Biograph
D.V. M., College of Veterinary Medicine of Beijing Agricultural University, Beijing, China. 1978/3-1982/12
Master, Science in Animal Histology and Embryology at Beijing Agricultural University, Beijing, China. 1982/8-1985/8
Ph. D, Molecular & Cell Biology at University of Florida, Gainesville, Florida. 1991/5-1996/5
 
Career
Research Assistant, College of Veterinary Medicine of Beijing Agricultural University, 1986-1987.
Assistant Professor, Animal Virology at Institute of Laboratory Animal Sciences of Beijing Agricultural University, 1987-1991.
Visiting Scientist, Swine Disease Unit of National Animal Disease Center, USDA, 1989.
Postdoctoral Research Fellow, Department of Medicine of University of Oklahoma Medical Center, 1996-1997.
Research Scientist, Howard Hughes Medical Institute and Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, 1997-2000.
Senior Scientist, Genzyme Corporation, 2000-2004
Professor,Director, the Center for Molecular Virology, Institute of Microbiology, Chinese Academy of Sciences, 2004-present.

Research Interests
Our research mainly focuses on following areas, such as the molecular mechanisms of interspecies transmission of avian influenza virus, molecular evolution, pathogenicity and epidemiology biology of animal RNA viruses, and development of new antiviral drugs.
1. Understanding the molecular mechanism of avian influenza virus (AIV) infection and determining the interaction between AIV matrix protein and human host factors.
2. Building research platform for understanding molecular evolution of animal RNA viruses, such as swine influenza virus, avian infectious bronchitis virus, avian infectious bursal disease virus and others.
3. Exploring and developing new type of antiviral drugs and immunostimulators including the interferon and small molecular therapeutic antibody.

Grants
1. “The interaction between pathogens and host/cellular factors” supported by the science supporting platform of the Ministry of Science and Technology P.R. China, 2006-2010.
2. “The pathogenesis of avian influenza virus and the development of new type nano-grade therapeutic antibody” supported by the 10th “five-year plan” project of Ministry of Science and Technology P.R. China, 2005-2007.
3. “Molecular mechanism of interaction between viral protein-host intracellular proteins” supported by the 973 Program of China, 2005-2010.
4. “China-Japan research collaboration on emerging and re-emerging Infections” supported by the Ministry of Science and Technology of P.R. China and the Institute of Medical Science, the University of Tokyo, 2006-2010.
5. “Pathogenic mechanism and immunological control on emerging and re-emerging pathogen” supported by the Ministry of Science and Technology of P.R. China and the Institute of Medical Science, the University of Tokyo, 2007-2010.
6. “The research and application of molecular biology of animal virus” supported by “One hundred talent program”, Chinese Academy of Sciences, 2007-2010.
7. “Interspecies transmission mechanism on avian influenza virus and Molecular mechanism of infectious pathogen” supported by Project Direction in Agricultural Sciences Section , Chinese Academy of Sciences. 2007-2010.
8. “Research and Demonstration on Rabies Virus Detection and Prevention Technology” supported by Public welfare projects of Ministry of Agriculture.2008-2010.
9. “Molecular Evolution Platform on Pathogen Virus Diseases” supported by the 10th “five-year plan” project of Ministry of Science and Technology P.R. China, 2006-2010. (Closed)

Awards and Honors
1. First place of Scientific Achievement Award of People’s Republic of China Ministry of Agriculture, 1990.
2. Japanese government scholarship 1991.
3. Associate Member of Sigma Xi Scientific Research Society since 1994.
4. National Institutes of Health scholarship, 1994.
5. National Institutes of Health young scientist grant, 1996.
6. American Society of Hematology junior research fellow award, 1997.
7. "One-Hundred Talent" Investigator, Chinese Academy of Sciences (CAS), 2004
8. Thomson Reuters Research Fronts Award, 2008

Patents
1. Feline Interferon ω and its Application. Patent No. ZL200510089864.6.
2. Swine Interferonγ and its Application. Patent No. 200710120248.1.
3. An Improved Swine Interferonγand its Application. Patent No. 200710176867.2.
4. Feline Interferonαits Application.Patent No. 200610112963.6.
5. An ELISA Kit for Rabies Virus Detection. Patent No. 200610113337.9.

Publications
1. Xiaoling Liu, Lei Sun, Maorong Yu, Zengfu Wang, Chongfeng Xu, Qinghua Xue, Ke Zhang, Xin Ye, Yoshihiro Kitamura, Liu-W-J *, 2009, Cyclophilin A interacts with influenza A virus M1 protein and impairs the early stage of the viral replication. Cellular Microbiology. (accepted)
2. L Sun, G-H Zhang, Y-L Shu, X-M Chen, Y-P Zhu, L-M Yang,G-P Ma, Yoshihiro Kitamura, Liu-W-J *.2009. Genetic correlation between H3N2 human and swine influenza viruses. Journal of Clinical Virology. 44:141-144.
3. Z-M Qin#, L Sun#, B-C Ma, Z-Z Cui, Y-P Zhu, Yoshihiro Kitamura, Liu-W-J *. 2008. F gene recombination between genotype II and VII Newcastle disease viru. Virus Research. 131:299–303.
4. L-T Tan, H-Y Xu, Y-L Wang, Z-M Qin*, Lei Sun, Liu-W-J , and Z-Z Cui. 2008. Molecular characterization of three new virulent Newcastle disease virus (NDV) variants isolated from China. Journal of Clinical Microbiology. 46(2):750-3
5. Z-M Qin*, L-T Tan, H-Y Xu, B-C Ma, Y-L Wang, X-Y Yuan, and Liu-W-J *. 2008. Pathotypical Characterization and Molecular Epidemiology of Newcastle Disease Virus Isolates from Different Hosts in China from 1996 to 2005. Journal of Clinical Microbiology. 46(2):601-11
6. 王鸿宾,贾晓娟,杨利敏,孙蕾,王红宁,刘文军*.2008. 新型猫源干扰素FeIFN-ω与干扰素FeIFN-α的表达及抗病毒活性比较. 生物工程学报.24(9):1556-1560.
7. L Sun#, G-H Zhang#, J-W Jiang, J-D Fu, T Ren, W-S Cao, C-A Xin, M Liao, Liu-W-J *. (2007). A Massachusetts Prototype like Coronavirus Isolated from Wild Peafowls is Pathogenic to Chickens. Virus Research. 130:121–128
8. L-M Yang* Q-H Xue,* L Sun, Y-P Zhu, and Liu-W-J . (2007). Cloning and Characterization of a Novel Feline IFN-ω. Journal of Interferon & Cytokine Research. 27:119–127
9. 吴静,杨利敏,施振声,黄立纲,刘文军*.(2007).ω-3多聚不饱和脂肪酸脱氢酶的真核表达及鉴定. 中国生物工程杂志. 27(10):44-47
10. 姚艳,张桂红,刘文军,陈铁桥,孙蕾*. (2007). 一株H3N2亚型猪流感病毒广东分离株的基因组序列分析. 微生物学报. 47(5): 805-809.
11. L-M Yang, L-Z Zhao, R-L Hu, Z-S Shi, and Liu-W-J *. (2006). A Novel Double-Antigen Sandwich Enzyme-Linked Immunosorbent Assay for Measurement of Antibodies against Rabies Virus. Clinical and Vaccine Immunology. 13(8):966-968
12. Liu, J., Xiao, H., Lei, F., Zhu, Q, Qin, K., Zhang, X., Zhang, X., Zhao, D., Wang Q., Feng, L., Ma, J., Liu-W-J , Gao, F.( 2005). Highly pathogenic H5N1 influenza virus infection in migratory birds. Science. 309:5738
13. Kamura, T., Burian, D., Liu, W-J., Khalili, H., Schmidt, S. L., Conard, M. N. , Conaway, R. C. , Conaway, J. W., and Shilatifard, A. (2000) Cloning and Characterization of ELL-associated Proteins EAP45 and EAP20: A Role for Yeast EAP-like Proteins in Regulation of Gene Expression by Glucose. Journal of Biological Chemistry. 276:16528.
14. Peltier, M.R., Liu. W-J. and Hansen, P. J. (2000) Regulation of Lymphocyte Proliferation by Uterine Serpin: CD25 Expression and Responsiveness to Interleukin-2. Proceedings of Society for Experimental Biology and Medicine. 223: 75.
15. Ramachdran, R., Nollert M. U., Qiu, H., Liu, W-J., Cummings, R. D., Zhu, C., and McEver, R. P. (1999) Tyrosine replacement in P-selectin glycoprotein ligand-1 affects distinct kinetic and mechanical properties of bonds with P-and E-selectin. Proceedings of National Academy of Sciences of the United States of America. 96:13771.
16. Liu, W.-J., Petier M.R .and Hansen, P. J. (1999) Binding of Ovine Uterine Serpin to Lymphocytes. American Journal of Reproductive Immunology. 41:428.
17. Liu, W.-J., Ramachdran, R., Kang, J., Cummings, R. D., McEver, R. P. (1998) Identification of N-terminal residues on P-selectin glycoprotein ligand-1 required for binding to P-selectin. Journal of Biological Chemistry. 273:7078.
18. Liu, W.-J., Gottshall, S. L., and Hansen, P. J. (1997) Increased expression of cell surface markers on / TCR+ intraepithelial lymphocytes by the local presence of the conceptus. American Journal of Reproductive Immunology. 37:199
19. Hansen, P. J and Liu, W.-J. (1996) Immunological aspects of pregnancy: concepts and speculations derived using the sheep as a model. Animal Reproduction Science. 42:483.
20. Liu, W.-J. (1996) Regulation of lymphocyte function by sheep endometrial serpin-like protein. Ph.D dissertation, University of Florida.
21. Liu, W.-J., and Hansen, P. J. (1995) Progesterone-induced secretion of a soluble form of dipeptidyl peptidase IV (CD26) by the uterine endometrium of the ewe and cow that costimulates lymphocyte proliferation. Endocrinology. 136:779.
22. Skopets, B., Liu, W.-J., and Hansen, P. J. (1995) Effects of endometrial serpin-like proteins on immune responses in sheep. American Journal of Reproductive Immunology. 33:86.
23. Hansen, P. J. and Liu, W.-J. (1994) Biochemical/physiological properties of endometrial serpin-like proteins. Advances in Contraceptive Delivery System. 10:339.
24. Liu, W.-J., and Hansen, P. J. (1993) Effect of the progesterone-induced serpin-like proteins of the sheep endometrium on natural-killer cell activity in sheep and mice. Biology of Reproduction. 49:1008.
25. Liu, W.-J., and Li, B. (1989) Morphological observation on the seminiferous epithelial development in the testis of the boar (in Chinese). Acta Veterinaria et Zootechnica Sinica. 19 (suppl. 1): 78.
26. Liu, W.-J., and Li, B. (1989) Ultrastructural studied on supporting cell development and establishment of the blood-testis barrier in the seminiferous tubules of the boar (in Chinese). Acta Veterinaria et Zootechnica Sinica. 19 (suppl. 1): 83.
27. Liu, W.-J., and Jia, W. (1988) Use of monoclonal antibody against Sendai virus or viral infection diagnosis (in Chinese). Acta Agriculturae Universitatis Pekinesis. 14:164.
28. Jia, W., and Liu, W.-J. (1988) Comparison of indirect ELISA with sandwich ELISA for diagnosis of mouse hepatitis virus (MHV) (in Chinese). Acta Agriculturae Universitatis Pekinesis. 14:273.
29. Liu, W.-J. (1988) The development of the immunoblot assay for diagnosis of Sendai virus infection in mice (in Chinese). Chinese Journal of Veterinary Medicine. 14:3.

Laboratory Staff
Dr. Yiping Zhu    Assistant Professor
Dr. Lei Sun      Assistant Professor
Limin Yang      Research Assistant
Xiaojuan Jia     Research  Assistant
Jiani Cai        Research  Assistant
Rongge Zhu      Technician
Xianhua Fu       Technician
Tingjian Liu       Technician
Xi Liang       Technician
Lin Liu       Secretary

Graduate Students
Zengfu Wang     (2005-2008)
Xiaoling Liu      (2005-2008)
Qinghua Xue    (2005-2008)
Maorong Yu    (2006-2009)
Shanshan Meng   (2006-2009)
Chongfeng Xu    (2006-2009)
Shuai Cao     (2007-2009)
Ke Zhang     (2005-2009)
Caiwei Chen    (2006-2011)
Shanshan Wang   (2006-2011)
Xuemei Chen    (2006-2011)
Zhendong Zhao   (2007-2012)
Shengyan Gao    (2007-2012)
Dingyong Zhang   (2007-2012)
Lu Lu       (2008-2013)
Zheng Li      (2008-2013)