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     Anaerobes Research Group

         Principal Investigator
         Dr. Prof. Xiuzhu Dong

        Tel/Fax: 86-10-64807413




Research Area

The laboratory is currently conducting the researches as follows: (i) physiology of rumen fibrolytic bacteria; (ii) cold-adapted methanogens that play a role in CH4 emission of the cold wetland, and their cold adaptive mechanisms; and (iii) antioxidative mechanisms of oral streptococci and the interspecies interaction with S. mutans.

Research Interests

Our current ongoing projects include: (i) quorum sensing regulation in the cellulase synthesis by Cellulosilyticum ruminicola H1; (ii) genome-wide post-transcription involved cold adaptive mechanisms of methanogenic archaea; and (iii) antioxidative mechanisms of Streptococcus oligofermentans.


Lab Members

Principal Investigator

Ph.D., Professor Xiuzhu Dong

- Bachelor of Agronomy, Beijing Agricultural University, Jan. 1982.
- Master of Science, Institute of Microbiology, Chinese Academy of Sciences. Oct. 1984. (Thesis: Taxonomy of acetic acid bacteria).
- Ph. D. Wageningen University, the Netherlands, Dec. 1994. (Thesis: Role of H2 and formate in the syntrophic degradation of butyrate and propionate).

Professional Experience
- Assistant researcher in Institute of Microbiology, Chinese Academy of Sciences. 1985-1989.
- Post-doctor in University of Alabama at Birmingham, USA, 1995-1996.
- Associate Professor in Institute of Microbiology, Chinese Academy of Sciences. 1996-1999.
- Professor in Institute of Microbiology, Chinese Academy of Sciences. 1999-

Editorial Board:
Editorial board of Frontier terrestrial microbiology
Associate chief editor of Acta Microbiologica Sinica
Editorial board of Microbiology

Permanent staff
Permanent staff
Huichun Tong

Ph.D Associate Professor

Jie Li

Ph.D Assistant Professor

Lingyan Li

M.Sc Research assistant

Postdoctoral Fellow
Dr. Yi Cao

Graduate Students
Zijuan Chen
Cold Adaption Mechanism of methanolobus psychrophilus R15

Liguang Zhou
Genetic Transformation System for Methanolobus Psychrophilus R15

Lei Qi
mRNA stability in the cold Adaptation of Methanolobus psychrophilus

Xin Zheng
Characterization of Methenolobus psychrophilus RNase J

Xinhui Wang
Mechanisms of Oxidative Stress defense in Streptococcus oligofermentans
Shanzheng Li
Quorum Sensing mediated cellulose production by Cellulosilyticum ruminicola

Bo Zhang
Cold adaptation mechanism of methanolobus psychrophilus R15

Former Graduate Students
Chunyang Zhang     2001-2004
Kai Ma                   2001-2006 
Chenggang Wu       2003-2006
Shuangya Chen       2002-2005
Lili Niu                   2004-2007
Wei Chen               2004-2007
Kegui Zhgang         2003-2008
Lei Song                2005-2008
Shichun Cai           2004-2009
Guishan Zhang       2006-2009
Jinxing Zhu            2005-2011
Jiabao Li               2007-2011
Peng Zhou            2008-2012
Xiaopeng Guo      2007-2011
Jun Cai                 2008-2013
Fan Zhang            2008-2011
Can Wang            2009-2012
Lei Liu                 2009-2012
Yue Wu              2009-2012

Recent publications

1. Guishan Zhang, Fan Zhang, Gang Ding, Jie Li, Xiaopeng Guo, Jinxing Zhu, Liguang Zhou, Shichun Cai, Xiaoli Liu, Yuanming Luo, Guifeng Zhang, Wenyuan Shi & Xiuzhu Dong*. Acyl homoserine lactone-based quorum sensing in a methanogenic archaeon. The ISEM J. 6(7):1336-44. 2012.
2. Fan Zhang, Xiaoli Liu, and Xiuzhu Dong*. Thermosyntropha tengcongensis sp. nov., a thermophilic bacterium that syntrophically degrades long-chain fatty acids. Int J Sys Evol Microbiol. 62: 759–763,2012.
3. Lei Liu, Huichun Tong, and Xiuzhu Dong. Function of pyruvate oxidase - lactate oxidase cascade in interspecies competition between Streptococcus oligofermentans and Streptococcus mutans. Appl Environ Microbiol 78: 2120-2127,2012.
4. Jinxing Zhu, Huajun Zheng, Guomin Ai, Guishan Zhang, Di Liu, Xiaoli Liu, and Xiuzhu Dong*. The genome characteristics and predicted function of methyl-group oxidation pathway in the obligate aceticlastic methanogens, Methanosaeta spp. PLoS ONE 7(5): e36756. 2012. (10.1371/journal.pone.0036756.)
5. Peng Zhou, Lei Liu, Huichun Tong*, Xiuzhu Dong*. Role of operon aaoso-mutt in antioxidant defense in Streptococcus oligofermentans. PLoS ONE. doi: 10.1371/journal.pone.0038133. 2012.
6. Jun Cai, Huichun Tong*, Xiuzhu Dong*. CcpA-dependent carbohydrate catabolite repression regulates galactose metabolism in Streptococcus oligofermentans. J Bacteriol. 194: 3824-3832. 2012.
7. Xiuzhu Dong*, Zijuan Chen. Psychrotolerant methanogenic archeae: diversity and cold adaptation mechanisms. Science China: Life Sciences. 55 (5): 1-7. 2012. (invited review)
8. Xin Dai, Yaxin Zhu, Yingfeng Luo, Lei Song, Di Liu, Li Liu, Furong Chen, Min Wang, Jiabao Li, Xiaowei Zeng, Zhiyang Dong, Songnian Hu, Lingyan Li, Jian Xu*, Li Huang*, Xiuzhu Dong*. Metagenomic insights into the fibrolytic microbiome in yak rumen. PLoS ONE 7(7): e40430. doi:10.1371/journal.pone.0040430. 2012.
9. Chen Z, Yu H, Li L, Hu S, Dong X*. The genome and transcriptome of a newly described psychrophilic archaeon, Methanolobus psychrophilus R15, reveal its cold adaptive characteristics. Environ Microbiol Report. 4(6), 633–641. 2012.
10. Jinxing Zhu, Xiaoli Liu and Xiuzhu Dong*. Methanobacterium mobile sp. nov. and Methanobacterium flexile sp. nov., isolated from the lake sediment of Qaidam Basin, China. Int J Sys Evol Microbiol, 61,2974- 2978. 2011.
11. Jiabao Li, Shichun Cai, Yuanming Luo, and Xiuzhu Dong. Three feruloyl esterases in Cellulosilyticum ruminocola H1 act synergistically to hydrolyze esterified polysaccharides. Appl Environ Microbiol. 77, 6141-6147. 2011.
12. Shichun Cai, Xin Zheng, and Xiuzhu Dong*. CBM3d, a novel sub-family of family 3 carbohydrate binding module identified in cel48a exoglucanase of Cellulosilyticum ruminicola. J Bacteriol. 193, 5199-5206, 2011.
13. Shichun Cai and Xiuzhu Dong*. Cellulosilyticum ruminicola gen. nov., sp. nov. isolated from the rumen of yak and reclassification of Clostridium lentocellum as Cellulosilyticum lentocellum comb. nov. Int J Syst Evol Microbiol, 60: 845 – 849.2010.
14. Na Jiang, Yanfen Wang, Xiuzhu Dong*. Methanol as the primary methanogenic and acetogenic precursor in the cold Zoige wetland at Tibetan Plateau. Microbial Ecol. 60: 206-213. 2010.
15. Jianqing Tian, Yanfen Wang, Xiuzhu Dong*. Methanoculleus hydrogenotrophus sp. nov., a novel methanogenic archaeon isolated from Tibetan Plateau in China. Int J Sys Evol Microbiol, 60: 2165 - 2169. 2010.
16. Kegui Zhang, Lei Song, Xiuzhu Dong*. Proteiniclasticum ruminis gen. nov., sp. nov., a strictly anaerobic proteolytic bacterium isolated from yak rumen. Int J Sys Evol Microbiol, 60:2221 - 2225, 2010.
17. Shichun Cai, Jiabao Li, Fen Ze Hu, Kegui Zhang, Yuanming Luo, Benjamin Janto, Robert Boissy, Garth Ehrlich*, Xiuzhu Dong*. Cellulosilyticum ruminicola, a newly described rumen bacterium that possesses redundant fibrolytic genes in the genome and degrades lignocellulose by multiple CBM-borne fibrolytic enzymes. Appl Environ Microbiol 76, 3818-3824, 2010.
18. J. Zhang, H.C. Tong, XZ. Dong, L. Yue, X.J. Gao. A preliminary study of biological characteristics of Streptococcus oligofermentans in Oral Microecology. Caries Res. 2010.
19. Xiuzhu Dong, The genus Acetanaerobacterium. Second Edition of Bergey's Manual of Systematic Bacteriology. Volume 3.
20. Lei Song and Xiuzhu Dong*. Hydrogenoanaerobacterium saccharovorans gen. nov., sp. nov., isolated from the H2-producing UASB granules. Int J Syst Evol Microbiol, 59: 295 - 299 .2009
21. Guishan Zhang, Jianqing Tian, Na Jiang, Xiaopeng Guo, Yanfen Wang, and Xiuzhu Dong*. Methanogen community in Zoige wetland of Tibetan plateau and phenotypic characterization of a dominant uncultured methanogen cluster ZC-I. Environ Microbiol. 10, 1850–1860. 2008.
22. Guishan Zhang, Na jiang, Xiaoli Liu, Xiuzhu Dong*. Methanogenesis from methanol at low temperature by a novel psychrophilic methanogen, Methanolobus psychrophilus sp. nov., prevalent in Zoige wetland of Tibetan plateau. Appl Environ Microbiol 74:6114–6120. 2008.
23. Huichun Tong, Wei Chen, Wenyuan Shi, Fengxia Qi, Xiuzhu Dong*. SO-LAAO, a novel L-amino acid oxidase that enables Streptococcus oligofermentans to over-compete Streptococcus mutans by generating H2O2 from peptone. J. Bacteriol. 190:4716-4721.2008.
24. Lei Song and Xiuzhu Dong. Clostridium amylolyticum sp. nov., isolated from the H2-producing UASB granules. Int J Syst Evol Microbiol 58: 2132-2135. 2008.
25. Sun lei, Song Wei*, Xiuzhu Dong*. Endophytic Bacterial Diversity in Rice (Oryza sativa L.) Roots Estimated by 16S rDNA sequence analysis. Microbial Ecology.55:415-424. 2008
26. Lili Niu,Lei Song and Xiuzhu Dong. Proteiniborus ethanoligenes gen. nov., sp. nov., an Anaerobic Protein-utilizing Bacterium Int J Syst Evol Microbiol 58: 12 – 16, 2008.
27. Huichun Tong, Wei Chen, Justin Merritt, Fengxia Qi, Wenyuan Shi, Xiuzhu Dong*. Streptococcus oligofermentans inhibits Streptococcus mutans through conversion of lactic acid into inhibitory H2O2: a possible counteroffensive strategy for inter-species competition. Mol. Microbiol. 63(3): 872-880,2007.
28. Chenggang Wu, Guishan Zhang, Xiaoli Liu and Xiuzhu Dong*. Bicarbonate is a stimulus in the inter-species induced sporulation of strict anaerobic Syntrophomonas erecta subsp. sporosyntropha. Extremophiles 11(6),827-832. 2007.
29. Tong Huichun; Zhu, Baoli; Chen, Wei; Qi, Fengxia; Shi, wenyuan; Dong, Xiuzhu* Establishing a genetic system for ecological studies of streptococcus oligofermentans. FEMS Microbiol. Let. 264:213–219, 2006.
30. Chenggang Wu,Xiaoli Liu and Xiuzhu Dong* Syntrophomonas cellicola sp. nov., a novel sporeforming syntrophic bacterium isolated from a distilled-spirit-fermenting cellar and assignment of Syntrophospora bryantii to Syntrophomonas bryantii sp. nov., comb. nov. Int. J. Syst. Evol. Microbiol. 56: 2331-2335.2006.
31. Kai Ma, Xiaoli Liu, Xiuzhu Dong* Methanosaeta harundinacea, sp. nov., a novel acetate-scavenging methanogen isolated from a UASB reactor. Int. J. Syst. Evol. Microbiol. 56: 127-131,2006.
32. Dengdi An, Shichun Cai and Xiuzhu Dong*. Actinomyces ruminicola sp. nov. isolated from cattle rumen Int. J. Syst. Evol. Microbiol. 56: 2043-2048,2006.
33. Chenggang Wu,Xiaoli Liu and Xiuzhu Dong* Syntrophomonas cellicola sp. nov., a novel sporeforming syntrophic bacterium isolated from a distilled-spirit-fermenting cellar and assignment of Syntrophospora bryantii to Syntrophomonas bryantii sp. nov., comb. nov. Int. J. Syst. Evol. Microbiol. 56: 2331-2335.2006.