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     Biomicrofluidics Group

         Principal Investigator
         Dr. Wenbin Du

     Tel/Fax:+86 (0) 10-8299 4175

       Lab: +86 (0) 10-8299 4195
             wenbin(at)im.ac.cn

 

 

Address: [map]
Institute of Microbiology
Chinese Academy of Science
No.7 Beitucheng West Road, Room G-303
Chaoyang District, Beijing 100029 China

 

Research Field

Microfluidics, Microbiology and Bioanalytical Chemistry

Research Interests

Our research spans a broad range of Microbiology, Microfluidics, and Bioengineering.  We are developing microfluidic tools to explore and understand the diversity and functions of microbial communities.  We use droplet microfluidics for high-throughput screening using single bacterial cells in nanoliter to picoliter volumes. Our goal is to realize single bacterial cell acquisition, cultivation, manipulation and identification in an integrated and automated workflow.  Using these platforms, we are interested in the isolation and detection of rare microbes and extremophiles from the environment, and we are also applying them on early diagnosis of rare pathogen infection.  We are also intested in further development of “Lab-on-a-Chip” systems for microbiological research, specifically in the integration of fluidic regulators, detectors, imaging systems, and interfacing microfluidic devices with instrumental analysis methods.

Our current ongoing projects include:

  1. Single cell isolation and cultivation in droplet array
  2. Bacterial chemotaxis in microfluidic devices
  3. isolation and multiplex PCR identification of bacterial cells from clinical samples
  4. Interface microfluidic macromolecules separation with MALDI-TOF MS

 


Lab Members

Principal Investigator

Wenbin Du, Ph.D.

Professor

Email: wenbin(at)im.ac.cn

 


Education
06/2002 B. Sc. in Chemistry (Zhejiang University, China)
09/2002-06/2007Ph. D. in Chemistry (Zhejiang University, China) with Prof. Zhaolun Fang and Prof. Qun Fang

Professional Experience
09/2007-08/2011 Postdoctoral Fellow (The University of Chicago) with Prof. Rustem F. Ismagilov
09/2011-03/2013 Principle Investigator, Renmin University of China, Department of Chemistry
03/2013-Present Professor, State Key Laboratory of Microbial Resources, The Institute of Microbiology, Chinese Academy of Sciences

 

Permanent staff


Peng Xu

Ph.D. assistant professor
Research field: Separation and analysis of biological macromolecules
Email: xup(at)im.ac.cn

 

Graduate Students

 

Dongyang Cai

Graduate student, 2011

Email: dongyangcai1988(at)163.com

 

Shujun Wang

Graduate student, 2011

Email: shujun_ruc(at)126.com

 

Libing Dong

Ph.D. student, 2012
Email: mengxiang17(at)163.com

 

Chaohua Shen

Graduate student, 2012
Email: xssch1(at)163.com

 

Zengjun Jin

Ph.D. student, 2013
Email: jinteng118(at)163.com

 

Mengyue Nie

Graduate student, 2013

hermione111(at)163.com

Qiaolian Yi
 
Undergraduate Student 2010
yqlotus(at)163.com


 


Selective Publications

(1) Shen, F.; Sun, B.; Kreutz, J. E.; Davydova, E. K.; Du, W. B.; Reddy, P. L.; Joseph, L. J.; Ismagilov, R. F.*, Multiplexed Quantification of Nucleic Acids with Large Dynamic Range Using Multivolume Digital RT-PCR on a Rotational SlipChip Tested with HIV and Hepatitis C Viral Load, Journal of the American Chemical Society 2011, 133, 17705.
(2) Pompano, R. R.; Liu, W. S.; Du, W. B.; Ismagilov, R. F.*, Microfluidics Using Spatially Defined Arrays of Droplets in One, Two, and Three Dimensions, Annual Review of Analytical Chemistry, Vol 4; Cooks, R. G. Y. E. S., Ed. 2011; Vol. 4, p 59.
(3) Kim, H. J.; Du, W. B.; Ismagilov, R. F.*, Complex function by design using spatially pre-structured synthetic microbial communities: degradation of pentachlorophenol in the presence of Hg(II), Integrative Biology 2011, 3, 126.
(4) Gu, S. Q.; Zhang, Y. X.; Zhu, Y.; Du, W. B.; Yao, B.; Fang, Q.*, Multifunctional Picoliter Droplet Manipulation Platform and Its Application in Single Cell Analysis, Analytical Chemistry 2011, 83, 7570.
(5) Du, W. B.; Sun, M.; Gu, S. Q.; Zhu, Y.; Fang, Q.*, Automated Microfluidic Screening Assay Platform Based on Drop Lab. Analytical Chemistry 2010, 82, 9941.
(6) Li, L.; Du, W. B.*; Ismagilov, R. F.*, Multiparameter Screening on SlipChip Used for Nanoliter Protein Crystallization Combining Free Interface Diffusion and Microbatch Methods, Journal of the American Chemical Society 2010, 132, 112.
(7) Li, L.; Du, W. B.; Ismagilov, R. F.*, User-Loaded SlipChip for Equipment-Free Multiplexed Nanoliter-Scale Experiments, Journal of the American Chemical Society 2010, 132, 106.
(8) Kreutz, J. E.; Shukhaev, A.; Du, W. B.; Druskin, S.; Daugulis, O.; Ismagilov, R. F.*, Evolution of Catalysts Directed by Genetic Algorithms in a Plug-Based Microfluidic Device Tested with Oxidation of Methane by Oxygen, Journal of the American Chemical Society 2010, 132, 3128.
(9) Shen, F.; Du, W. B.; Davydova, E. K.; Karymov, M. A.; Pandey, J.; Ismagilov, R. F.*, Nanoliter Multiplex PCR Arrays on a SlipChip, Analytical Chemistry 2010, 82, 4606.
(10) Shen, F.; Du, W. B.; Kreutz, J. E.; Fok, A.; Ismagilov, R. F.*, Digital PCR on a SlipChip, Lab on a Chip, 2010, 10, 2666.
(11) Liu, W. S.; Chen, D. L.; Du, W. B.; Nichols, K. P.; Ismagilov, R. F.*, Slip Chip for Immunoassays in Nanoliter Volumes, Analytical Chemistry 2010, 82, 3276.
(12) Du, W. B.; Li, L.; Nichols, K. P.; Ismagilov, R. F.*, SlipChip, Lab on a Chip 2009, 9, 2286.
(13) Zhang, T.; Fang, Q.*; Du, W. B.; Fu, J. L., Microfluidic Picoliter-Scale Translational Spontaneous Sample Introduction for High-Speed Capillary Electrophoresis, Analytical Chemistry 2009, 81, 3693.
(14) Liu, W.; Kim, H. J.; Lucchetta, E. M.; Du, W. B.; Ismagilov, R. F.*, Isolation, incubation, and parallel functional testing and identification by FISH of rare microbial single-copy cells from multi-species mixtures using the combination of chemistrode and stochastic confinement, Lab on a Chip 2009, 9, 2153.
(15) Chen, D. L.†; Du, W. B.† (equal contribution); Liu, Y.†; Liu, W.†; Kuznetsov, A.; Mendez, F. E.; Philipson, L. H.; Ismagilov, R. F.*, The chemistrode: A droplet-based microfluidic device for stimulation and recording with high temporal, spatial, and chemical resolution, Proceedings of the National Academy of Sciences of the United States of America 2008, 105, 16843.
(16) Du, W. B.; Fang, Q.*; Fang, Z. L., Microfluidic sequential injection analysis in a short capillary, Analytical Chemistry 2006, 78, 6404.
(17) Sun, M.; Du, W. B.; Fang, Q.*, Microfluidic liquid-liquid extraction system based on stopped-flow technique and liquid core waveguide capillary, Talanta 2006, 70, 392.
(18) Du, W. B.; Fang, Q.*; He, Q. H.; Fang, Z. L., High-throughput nanoliter sample introduction microfluidic chip-based flow injection analysis system with gravity-driven flows, Analytical Chemistry 2005, 77, 1330.
(19) He, Q. H.; Fang, Q.; Du, W. B.; Huang, Y. Z.; Fang, Z. L., An automated electrokinetic continuous sample introduction system for microfluidic chip-based capillary electrophoresis, Analyst 2005, 130, 1052.
(20) Du, W. B.; Fang, Q.*; Fang, Z. L., A microfluidic chip for absorbance measurements with long optical path-length based on a liquid-core waveguide technique, Chemical Journal of Chinese Universities-Chinese 2004, 25, 610.
(21) Fang, Q.*; Du, W. B.; He, Q. H.; Fang, Z. L., High throughput sample introduction system for microfluidic chips, Chemical Journal of Chinese Universities-Chinese 2004, 25, 1442.