Bacterial Antibiotic Resistance Mechanisms Laboratory

Principal Investigator
Dr. Assoc. Prof. Jie Feng

Tel:0086-10-64807802
E-mail: fengj@im.ac.cn

Research Area

Mechanisms of antibiotic Resistance in bacteria.

Research Interests

Our group is committed to investigating the novel acquired and intrinsic resistance genes in bacterial pathogens, as well as in environmental bacteria. We are also trying to explore the mobility of novel mobile elements, to uncover the mechanism of dissemination of resistance genes. We believe that our studies will substantially contribute to understanding of the evolution and epidemiology of resistance. The researches in our group are currently focused on the development of resistance, including: 1) Acquired resistance, we identified a number of novel acquired resistance genes and mobile elements unraveling the way that bacteria develop resistance; 2)Intrinsic resistance, we characterize the novel intrinsic resistance genes in Bacillus and Acinetobacter, and demonstrated that the intrinsic resistance genes can be the origin of acquired resistance genes. 3) Diagnosis of phenotype and genotype of resistant bacteria

Lab Members

Jie Feng, Ph.D.,Principal Investigator, Associate Professor, fengj@im.ac.cn

Education

    2002-2006  Ph.D (IMCAS), Identfication of a novel maleylpyruvate isomerase that needs mycothiol as cofactor and links mycothiol biosynthesis and gentisate assimilation in Corynebacterium glutamicum.

Professional Experience

    2009-Present   Principal Investigator, Associate Professor, Institute of Microbiology, Chinese Academy of Sciences (IMCAS)

    2006-2009   Postdoc at Infectious Disease Research Center, University, Canada, Genome sequencing and proteomic and transcriptomic analysis revealed novel mechanism of linezolid resistance in Streptococcus pneumonia, supported by CIHR grant


Recent Publications

1 Xu Yixiang, Wang Chao, Zhang Gang, Tian Jingjing, Liu Ying, Shen Xihui, Feng Jie*. ISCR2 is associated with the dissemination of multiple resistance genes among Vibrio spp. and Pseudoalteromonas spp. isolated from farmed fish. Arch Microbiol. 2017 Mar 29. doi: 10.1007/s00203-017-1365-2.
2  Zhang Gang, Leclercq Sebastien Olivier, Tian Jingjing, Wang Chao, Yahara Koji, Ai Guomin, Liu Shuangjiang, Feng Jie*. A new subclass of intrinsic aminoglycoside nucleotidyltransferases, ANT(3")-II, is horizontally transferred among Acinetobacter spp. by homologous recombination. PLoS Genet. 2017, 13(2)
3 Leclercq Sebastien Olivier, Wang Chao, Sui Zhihai, Wu Hai, Zhu Baoli, Deng Ye, Feng Jie*.A multi-player game: Clostridium, Acinetobacter, and Pseudomonas species drive the antibiotic-resistance gene persistence in manured soils.Environ Microbiol. 2016, 18(10):3494-3508.
4 Leclercq Sébastien Olivier, Wang Chao, Zhu Yaxin, Wu Hai, Du Xiaochen, Liu Zhipei, Feng Jie*. Diversity of the Tetracycline Mobilome Within a Chinese Pig Manure Sample. Appl Environ Microbiol. 2016, 82(21):6454-6462.
5 Zhang Gang, Tian Jingjing, Wang Chao, Chen Jifeng, Feng Jie*. Identification of novel cryptic aminoglycoside phosphotransferases in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 2016, 60(11):6983-6985.
6 Zhang Gang, Wang Chao, Sui Zhihai, Feng Jie*. Insights into the evolutionary trajectories of fluoroquinolone resistance in Streptococcus pneumoniae. J Antimicrob Chemother. 2015, 70(9): 2499-506.
7 Wang Chao, Sui Zhihai, Sébastien Olivier Leclercq, Zhang Gang, Zhao Meilin, Chen Weiqi,Feng Jie*,Functional characterization and phylogenetic analysis of acquired and intrinsic macrolide phosphotransferases in the Bacillus cereus group. Environ Microbiol. 2015, 17(5): 1560-73.
8 Zhou Wenqing, Yao Kaihu, Zhang Gang, Yang Yonghong, Li Yun, Lv Yuan, Feng Jie*.  Mechanism for transfer of transposon Tn2010 carrying macrolide resistance genes in Streptococcus pneumoniae and its effects on genome evolution. J Antimicrob Chemother. 2014, 69(6): 1470-3.
9 Yang Jing, Wang Chao, Wu Jinyu, Liu Li, Zhang Gang, Feng Jie*. Characterization of a multiresistant mosaic plasmid from a fish farm sediment Exiguobacterium sp. isolate reveals aggregation of functional clinic-associated antibiotic resistance genes. Appl Environ Microb. 2014, 80(4): 1482-8.
10 Yang Jing, Wang Chao, Shu Chang, Liu Li, Geng Jianing, Hu Songnian, Feng Jie*. Marine sediment bacteria harbor antibiotic resistance genes highly similar to those found in human pathogens. Microb Ecol. 2013, 65(4): 975-81.
11 Zhang Gang, TianWenyu, Wang Chao, Feng Jie*. Identification of a novel resistance mutation in parE that confers high-level resistance to moxifloxacin in Streptococcus pneumonia. J Antimicrob Chemother. 2012, 67(11): 2773-4.
12 Feng Jie, Billal Dewan S, Lupien Andréanne, Racine Gina, Winstall Eric, Légaré Danielle, Leprohon Philippe, Ouellette Marc. Proteomic and Transcriptomic Analysis of Linezolid Resistance in Streptococcus pneumonia. J Proteome Res. 2011, 10: 4439–52.
13 Feng Jie, Lupien Andréanne, Hélène Gingras, Jessica Wasserscheid, Ken Dewar, Danielle Légaré, Marc Ouellette. Genome sequencing of linezolid resistant Streptococcus pneumoniae mutants reveals novel mechanisms of resistance. Genome Res. 2009, 19: 1214-23.